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[7d] "Evaluation of the health risks associated with cannabis use"
The evaluation of health risks associated with using, consuming, or being in the vicinity of cannabis is undertaken according to the following methodologies.
Cannabis Use Methods and Measures:
– a survey
– a focus group study
– self assessment questionnaire
Marijuana and THC Content Data (M&Ts):
– M&T samples are tested in order to test for THC content using HPLC, GC-TMS or GC-UV
• M&Ts are tested for THC content using two different methods (see "Methodological Details" below).
– The method using GC-MS/HPLC/HRT-MS is analytical of choice for the determination THC in M&Ts.
– THC is identified as the primary active constituents after isolation of THC and CBN identification its conjugates metabolites.
– A standard cannabis plant (a "seedling/leaf") which contained high levels of THC was used.
Methodological Details:
– Results were summarized and analyzed using either a focus group or survey.
– The results of surveys or focus groups were analyzed by using a standard survey instrument (which includes various questions about risk factors), followed by an analysis of quantitative results in terms number of participants, mean and standard deviation, differences between participants and non-participants (n = 15) at age 13.
– Questionnaires were presented to study subjects under observation in a private space, or, if appropriate, a quiet, public place (eg, room inside a research building).
– Written informed consent was obtained from those who consented to participate (the adolescent participants at age 13), and the ethical commission of research ethics committee, as well written informed consent for parents of adolescents (aged 12-15 at the time of first survey at age 14 and 15). Consent for participants aged above 15 was obtained from their parents.
– All data are analysed from the results of first survey at age 13 to 15.
Participants were also presented with a questionnaire on their use of cannabis products.
Drug testing: THC, CBD, delta-9-tetrahydrocannabinol ("THC-COOH"), cannabinol ("cannabidiol") and tetrahydrocannabinol ("THC-Tetrahydrocannabinol") was determined in the urine and M& Ts of 15 volunteers who were participants in the study. THC and other cannabinoids were also determined from saliva specimens. THC was measured by HPLC using 0.2 nL (or approximately 20 ng) of dried plant Eriacta 60 Pills 1mg $230 - $3.83 Per pill material, following the procedure outlined in International Conference on Harmonisation Methodology for Analytical Techniques. This method uses the separation of a specific cannabinoid, Δ9-tetrahydrocannabinol (THC), from the other major cannabinoids: cannabidiol (CBD) and cannabinol (CBN), as described by the International Agency for Research on Cancer (IARC) in its monographs. All cannabis used in the study was supplied by Netherlands Organization for Applied Scientific Research (TNO) under the authority of Research Ethics Committee on Medical Ethical Aspects in Human Research.
Results (n = 15 participants):
Frequency of cannabis use was not associated with age or sex.
In total, 25 of the participants reported having used cannabis in the last month.
Cannabis use was also not associated with any demographic or clinical risk factors.
In the focus groups or survey (n = 15 participants), individuals identified a positive association between their use and being "high status" or a criminal in their country. The positive Over the counter kamagra oral jelly association disappeared when cannabis use during adolescence was taken into account (n = 4 participants).
Discussion: Cannabis use, regardless of how often, is a risk factor for negative consequences associated with being an adult in a society where marijuana use is widely legal and accessible.
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